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a person achievable rationalization with the enhanced Aβ production in the Aph1 L30F/T164A mutant is that the mutations improve advanced formation. to check whether or not the Aph1 L30F/T164A mutants form a posh with other components, γ-secretase in yeast microsomes was immunoprecipitated using a PS1 antibody against the loop region (GIL3) (Figure 5).
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This study exposed that Aph1aL may be actively associated with γ-secretase catalytic activity. We analyzed the production of unique Aβ species and located that the ratio of extended Aβ species (Aβ42 and Aβ43) with Aβ40 was equivalent amongst the Aph1aL WT and L30F/T164A double mutant.
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